Anti-CD146  polyclonal antibody  (Rabbit)                   Catalog:0806-5       

Product Description: CD146 is a cell surface glycoprotein, originally identified as a melanoma antigen, whose expression is associated with tumor progression and the development of metastatic potential. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium.

Species Cross-reactivity:  Mouse 

Formulation:   1 mg/ml.

Application(s):   WB: 1:500

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on  melanoma using anti-CD146 polyclonal antibody

 

References:
1. Lehmann J.M., Riethmueller G., Johnson J.P.; "MUC18, a marker of tumor progression in human melanoma, shows sequence similarity to the neural cell adhesion molecules of the immunoglobulin superfamily."; Proc. Natl. Acad. Sci. U.S.A. 86:9891-9895(1989).
2. Sers C., Kirsch K., Rothbaecher U., Riethmueller G., Johnson J.P.; "Genomic organization of the melanoma-associated glycoprotein MUC18: implications for the evolution of the immunoglobulin domains."; Proc. Natl. Acad. Sci. U.S.A. 90:8514-8518(1993).