Anti-CD24    polyclonal antibody  (Rabbit)               Catalog:0804-4   

Product Description: The human CD24 antigen is a small heavily glycosylated, glycosylphosphatidylinositol (GPI)linked cell surface protein. It consists of a small protein core comprising 27 amino acids, which is extensively glycosylated and is bound to the membrane via a phosphatidylinositol anchor. The human CD24 antigen is physiologically expressed in developing or regenerating tissue, as well as in granulocytes, keratinocytes and renal tubular epithelium. It modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor.

Species Cross-reactivity:  Human

Formulation:   0.5 mg/ml

Application(s):   WB: 1:100-500       ICC: 1:250             IHC: 1:100-1:200

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on human spleen using anti-CD24 polyclonal antibody
 
References:
1. Kay R, Rosten PM, Humphries RK. CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol 1991; 147: 1412-1416
2. Aigner S, Ruppert M, Hubbe M, Sammar M, Sthoeger Z, Butcher EC, Vestweber D, Altevogt P. Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin. Int Immunol 1995; 7: 1557-1565