Anti-CD3 zeta   polyclonal antibody  (Rabbit)                       Catalog:0805-7         

Product Description: CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs.

Species Cross-reactivity:  Human , Mouse , Rat

Formulation:   0.5 mg/ml

Application(s):   WB: 1:1,000

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on Jurkat cell lysates using anti- CD3 zeta polyclonal antibody

 

References:
1.Weissman A.M., Hou D., Orloff D.G., Modi W.S., Seuanez H., O'Brien S.J., Klausner R.D.;"Molecular cloning and chromosomal localization of the human T-cell receptor zeta chain: distinction from the molecular CD3 complex.";Proc. Natl. Acad. Sci. U.S.A. 85:9709-9713(1988).
2.Rieux-Laucat F., Hivroz C., Lim A., Mateo V., Pellier I., Selz F., Fischer A., Le Deist F.;"Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.";N. Engl. J. Med. 354:1913-1921 (2006).