Anti-CD 80/B7-1  polyclonal antibody  (Rabbit)             Catalog0108-7

Product Description: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 is thought to play a role in the process of T-cell mediated killing.

Species Cross-reactivity:  Mouse

Formulation:   0.45 mg/ml

Application(s):   WB: 1:500

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on mouse Thymus using anti-CD8  polyclonal antibody

 

References:
1.Liaw C.W., Zamoyska R., Parnes J.R.; "Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene."; J. Immunol. 137:1037-1043(1986).
2.Youn H.J., Harriss J.V., Gottlieb P.D.; "Nucleotide sequence analysis of the C.AKR Lyt-2a gene: structural polymorphism in alleles encoding the Lyt-2.1 T-cell surface alloantigen."; Immunogenetics 28:345-352(1988).
3.Zamoyska R., Vollmer A.C., Sizer K.C., Liaw C.W., Parnes J.R.; "Two Lyt-2 polypeptides arise from a single gene by alternative splicing patterns of mRNA."; Cell 43:153-163(1985).