Anti-IL-7R-alpha (CD127 or CDw127), polyclonal antibody  (Rabbit)               Catalog0804-9                        

Product Description: The IL-7 cytokine plays a major role in the in vivo maintenance of polyclonal naive and memory T cells, positively regulating the survival, differentiation and proliferation of thymocyte and peripheral T lymphocyte populations. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor γ chain common to the IL-2, IL-4,IL-7, IL-9 and IL-15 receptors. Soluble forms of the IL-7 Rα have been reported.

Species Cross-reactivity:  Human

Formulation:   0.43mg/ml

Application(s):    WB:1:1000 - 1:5000 

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

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Fig: Western blot analysis on K562(A) and human skin tissue (B)using anti- IL-7R-alpha polyclonal antibody

 

References:
1. Yu L.-R., Zhu Z., et al. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."; J. Proteome Res. 6:4150-4162(2007).
2. Roifman C.M., Zhang J., et al. "A partial deficiency of interleukin-7R alpha is sufficient to abrogate T-cell development and cause severe combined immunodeficiency."; Blood 96:2803-2807(2000).
3. Gregory S.G., Schmidt S., Seth P., et al. "Interleukin 7 receptor alpha chain (IL7R) shows allelic and functional association with multiple sclerosis."; Nat. Genet. 39:1083-1091(2007).