Anti-Kinase D-interacting substrate 220, Mouse monoclonal IgG1           CatalogM0910-4                       

Product Description: Kinase D-interacting substrate 220 (Kidins220), also known as ARMS (ankyrin-rich membrane spanning) is an integral plasma membrane protein predominantly expressed in brain and neurons that presents a polarized location in neural cells, concentrating at the tip of extending neurites and in plasma membrane micro-domains corresponding to lipid rafts. The primary amino acid sequence of Kidins220 reveals 11 ankyrin repeats, 4 trans-membrane domains, a proline-rich region, a sterile-α motif (or SAM) domain, and a type I postsynaptic density-95/discs large/zonula occludens-1 (PDZ)-binding motif at the very C-terminus.

Species Cross-reactivity:  Mouse 

Formulation:   0.28 mg/ml

Application(s):    WB 1:500~1:1,000   ICC 1:100~1:500

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

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Fig: Western blot analysis on recombinant protein of Kidins220, the detected band is 48kDa.

 

References:
1." Identification and cloning of Kidins220, a novel neuronal substrate of protein kinase D.” Iglesias T, Cabrera-Poch N, Mitchell MP, Naven TJ, Rozengurt E, Schiavo G. J Biol Chem. 22;275(51):40048-56(2000).
2."Large-scale phosphorylation analysis of mouse liver."Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007)