Anti-SCA-1 polyclonal antibody
an 18 kDa phosphatidylinositol-anchored protein, is a member of
the Ly-6 antigen family. Sca-1 is the most recognized HSC marker
in mice with both Ly-6 haplotypes as it is expressed on
multipotent HSCs. Sca-1+ HSCs can be found in the adult bone
marrow, fetal liver and mobilized peripheral blood and spleen
within the adult animal. An anti-Sca-1 Ab is frequently used in
combination with negative selection for expression of a number of
cell surface markers characteristic of differentiated cells of
hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.
Species Cross-reactivity: Mouse
Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol.
Preservative: 0.05% Sodium Azide.
Storage Instruction: Store at +4°C after thawing.
Aliquot store at -20°C or -80°C. Avoid repeated freeze /
Purity: Immunogen affinity purified
Fig: Western blot analysis on Mouse
liver using anti-SCA-1 polyclonal antibody
1.Khan K., Dad Lindwall G., Maher S.E., Bothwell A.L.M.;
"Characterization of promoter elements of an
interferon-inducible Ly-6E/A differentiation antigen,
which is expressed on activated T cells and
hematopoietic stem cells."; Mol. Cell. Biol.
2.van de Rijn M., Heimfeld S., Spangrude G.J., Weissman
I.L.; "Mouse hematopoietic stem-cell antigen Sca-1
is a member of the Ly-6 antigen family."; Proc.
Natl. Acad. Sci. U.S.A. 86:4634-4638(1989).
3.van Bragt M.P., Ciliberti N., Stanford W.L., de Rooij
D.G., van Pelt A.M.; "LY6A/E (SCA-1) expression in
the mouse testis."; Biol. Reprod. 73:634-638(2005).