Anti-SCA-1 polyclonal antibody  (Rabbit)               Catalog:0804-10 

Product Description: Sca-1, an 18 kDa phosphatidylinositol-anchored protein, is a member of the Ly-6 antigen family. Sca-1 is the most recognized HSC marker in mice with both Ly-6 haplotypes as it is expressed on multipotent HSCs. Sca-1+ HSCs can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen within the adult animal. An anti-Sca-1 Ab is frequently used in combination with negative selection for expression of a number of cell surface markers characteristic of differentiated cells of hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.

Species Cross-reactivity:  Mouse 

Formulation:   0.52 mg/ml

Application(s):   WB: 1:1,000

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on Mouse liver using anti-SCA-1 polyclonal antibody

 

References:
1.Khan K., Dad Lindwall G., Maher S.E., Bothwell A.L.M.; "Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells."; Mol. Cell. Biol. 10:5150-5159(1990).
2.van de Rijn M., Heimfeld S., Spangrude G.J., Weissman I.L.; "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family."; Proc. Natl. Acad. Sci. U.S.A. 86:4634-4638(1989).
3.van Bragt M.P., Ciliberti N., Stanford W.L., de Rooij D.G., van Pelt A.M.; "LY6A/E (SCA-1) expression in the mouse testis."; Biol. Reprod. 73:634-638(2005).