Anti-Alkaline Phosphatase   polyclonal antibody  (Rabbit)     Cat #:   0806-4 

Product Description: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Placental ALP is highly polymorphic, there are at least three common alleles. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Species Cross-reactivity:  Human , Mouse , Rat

Formulation:   0.47 mg/ml.

Application(s):   WB: 1:500

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on Hela using anti-ALP polyclonal antibody

 

References:
1.Ezra E., Blacher R., Udenfriend S.; "Purification and partial sequencing of human placental alkaline phosphatase."; Biochem. Biophys. Res. Commun. 116:1076-1083(1983).
2.Garattini E., Hua J.-C., Pan Y.C.E., Udenfriend S.; "Human liver alkaline phosphatase, purification and partial sequencing: homology with the placental isozyme."; Arch. Biochem. Biophys. 245:331-337(1986).