Anti-c-kit (CD117)  polyclonal antibody  (Rabbit)               Catalog:0108-4

Product Description: CD117 Antigen, also called KIT or C-kit receptor, is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.

Species Cross-reactivity:  Human , Mouse , Rat

Formulation:   0.5 mg/ml

Application(s):   WB: 1:500, ICC: 1:100

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on jurkat cell lysates using anti-c-kit polyclonal antibody

 

References:
1. Yarden, Y., et al. (1987) EMBO (Eur. Mol. Biot. Organ.) J. 6:3341-3351.
2. Qiu, F. H.,et al. (1988) EMBO (Eur. Mol. Biot. Organ.) J. 7:1003-1011.
3. Besmer, et al. 1986. Nature (Lond.). 320:415-421.
4. Zsebo, K. M., et al. (1990) Cell. 63:195-201.
5. Spritz RA, Giebel LB, Holmes SA. (1992) Am J Hum Genet. Feb;5 (2):261-9.