Anti-C-myc HRP Conjugated, polyclonal antibody  (Rabbit)               Catalog:0912-4

Product Description: Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This c-Myc antibody detects endogenous levels of total c-Myc protein and is also for detection of Myc-tagged fusion protein.

Species Cross-reactivity:  Human

Formulation:   0.57mg/ml

Application(s):     WB: 1:1,000-1:2,000

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA.
 
Storage Instruction: Store at +4°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

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Fig1: Western blot analysis on myc-tagged recombinant protein using c-myc antibody HRP conjugated.

 

References:
1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
2. "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci. U.S.A. 90:3216-3220(1993)