polyclonal antibody (Rabbit)
gene encodes for a transcription factor that is believed to
regulate expression of 15% of all genes through binding on
Enhancer Box sequences (E-boxes) and recruiting histone
acetyltransferases (HATs). c-Myc is commonly activated in a
variety of tumor cells and plays an important role in cellular
proliferation, differentiation, apoptosis and cell cycle
progression. This c-Myc antibody detects endogenous levels of
total c-Myc protein and is also for detection of Myc-tagged fusion
Species Cross-reactivity: Human
WB: 1:500 ICC: 1:100-1:200
Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol.
Preservative: 0.05% Sodium Azide.
Storage Instruction: Store at +4°C after thawing.
Aliquot store at -20°C or -80°C. Avoid repeated freeze /
Purity: Immunogen affinity purified
Western blot analysis on myc-tagged
recombinant protein with (A) and A549 cell lysates
Fig2: ICC/IF image of c-myc antibody
stained human A549 cells. The secondary antibody
(green) was goat anti-rabbit IgG (H+L) FITC
1. "A quantitative atlas of mitotic
phosphorylation."Dephoure N., Zhou C., Villen J.,
Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc.
Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
2. "Transactivation of gene expression by Myc is inhibited
by mutation at the phosphorylation sites Thr-58 and
Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci.