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Cell Biology Service

 

Product Price (US $)
Generation of CHO stable cell lines expressing scFv-Fc or whole IgGs $8000
Construction of single-chain antibodies (scFv-Fc) + Generation of CHO stable cell

lines expressing scFv-Fc

$10000
Construction of whole IgGs + Generation of CHO stable cell lines expressing whole

IgGs

$10000
Cloning and sequencing hybridoma antibody heavy and light chain variable regions +

antigen-antibody binding confirmation using scFv + Generation of CHO stable cell

lines expressing scFv-Fc

$12000
Cloning and sequencing hybridoma antibody heavy and light chain variable regions +

antigen-antibody binding confirmation using whole IgGs + Generation of CHO stable

cell lines expressing whole IgGs

$12000
Construction of single-chain antibodies (scFv-Fc) $3500
Construction of whole IgGs $5500
Gene Synthesis and Subcloning + Transient Expression Evaluation + Stable

Mammalian Cell Line Development

$7000
Transient Expression Evaluation + Stable Mammalian Cell Line Development $5000

Note:

1,Price/availability/specifications subject to change without notice. If not specified, all products and services are for in vitro research use only. Not for use in or on humans or animals.

2, Before you send your order, please send us an email, let us know your requirement and what you should prepare.

Time needed:
1 week for synthesizing antibody variable regions.
2-3 weeks for cloning and sequencing antibody variable regions of hybridoma cell lines.
2-3 weeks for molecular construction of IgG or scFv-Fc.
1 week for assessment of the binding affinity and specificity of IgG or scFv.
2-3 weeks for generation of CHO stable cell lines expressing scFv-Fc or whole IgGs.

Experienced scientists working on antibody services for decades.
Description:

Cloned or synthesized antibody variable regions will be cloned into proprietary vectors for construction of scFv-Fc (human or mouse Fc) or whole IgGs (human constant domain or mouse constant domain), which have a heavy chain and a light chain. CHO stable cell lines expressing scFv-Fc or whole IgGs will be generated.

If requested, the antibody variable regions (including both VL and VH) of hybridoma cell lines will be cloned and sequenced. Antigen-antibody binding affinity will be confirmed by transiently expressing single-chain antibodies (scFv) or whole IgGs in mammalian expression systems and testing whether the recombinant scFv or antibodies retain the immunological activities of their corresponding parent monoclonal antibodies.

1. Construction of single-chain antibodies (scFv):

Connect VL with VH using a peptide linker;
Subclone into a proprietary vector;
Express recombinant scFv-Fc in mammalian expression systems;
Test whether the recombinant scFv-Fc retain the immunological activities of their corresponding parent monoclonal antibodies.
Deliverable: scFv-Fc DNA and purified scFv-Fc protein (100 ug; inquire if you need more).

2. Construction of whole IgGs (human or mouse constant region IgG1):

Fuse VL and VH into human or mouse IgG constant region backbone in mammalian expression vectors;
Express recombinant antibody in mammalian expression systems;
Purify IgG and test whether the recombinant antibody retain the immunological activities of their corresponding parent monoclonal antibodies.
Deliverable: whole IgG DNA and purified antibody (100 ug; inquire if you need more).

3. Stable cell line development using mammalian cells:

Transfection into mammalian cells and screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. The standard stable cell line generation service by default uses monolayer CHO cells and expression vector with the zeocin selectable marker. Clones will be screened using ELISA, Western, or cell-based assay of your interest. Our faster, more predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity.
Deliverable:
a. 10 individual clones.
b. Stable cell line development report.

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