Anti-Frizzled-5, polyclonal antibody  (Rabbit)                 Catalog0905-3

Product Description: Frizzled-5 belongs to the G-protein coupled receptor Fz/Smo family, contains 585 amino acids. It is localized at the plasma membrane and also found at the Golgi. Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.

Species Cross-reactivity:  Human

Formulation:   0.62 mg/ml

Application(s):    WB:1:500 - 1:1,000   ICC: 1:100-1:200

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

.  

Fig1: Western blot analysis on human ES cell using anti-Frizzled-5 polyclonal antibody.

 

References:
1. "A member of the Frizzled protein family mediating axis induction by Wnt-5A."He X., Saint-Jeannet J.P., Wang Y., Nathans J., Dawid I., Varmus H.Science 275:1652-1654(1997)
2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.Wilson R.K.Nature 434:724-731(2005)