Anti-Frizzled-5, polyclonal antibody  (Rabbit)                 Catalog0905-3

Product Description: Frizzled-5 belongs to the G-protein coupled receptor Fz/Smo family, contains 585 amino acids. It is localized at the plasma membrane and also found at the Golgi. Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.

Species Cross-reactivity:  Human

Formulation:   0.62 mg/ml

Application(s):    WB:1:500 - 1:1,000   ICC: 1:100-1:200

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity: Immunogen affinity purified


Fig1: Western blot analysis on human ES cell using anti-Frizzled-5 polyclonal antibody.


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