Anti-Oct-4 Mouse monoclonal IgG1          Catalog:M1003-6

Product Description: Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.

Species Cross-reactivity:  Human , Mouse , Rat

Formulation:   0.31 mg/ml

Application(s):   WB: 1:2,000   ICC: 1:50

Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
 
Storage Instruction: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
 
Purity: Immunogen affinity purified

  

Fig: Western blot analysis on D3 cell lysates using anti-Oct 4 monoclonal antibody.

 

References:
1."Oct4 pseudogenes are transcribed in cancers."Suo G., Han J., Wang X., Zhang J., Zhao Y., Zhao Y., Dai J.Biochem. Biophys. Res. Commun. 337:1047-1051(2005)
2."Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation."Wei F., Schoeler H.R., Atchison M.L.J. Biol. Chem. 282:21551-21560(2007)
3."Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1."Zhang Z., Liao B., Xu M., Jin Y.FASEB J. 21:3042-3051(2007)