Mouse monoclonal IgG1 Catalog：M1003-6
Description: Oct-4 (POU5F1) is transcription factor
that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a
trimeric complex with SOX2 on DNA and controls the expression of a
number of genes involved in embryonic development such as YES1,
FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis
and for embryonic stem cell pluripotency. It is expressed in
developing brain and highest levels also found in specific cell
layers of the cortex, the olfactory bulb, the hippocampus and the
Species Cross-reactivity: Human , Mouse , Rat
WB: 1:2,000 ICC: 1:50
Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol.
Preservative: 0.05% Sodium Azide.
Storage Instruction: Store at +4°C after thawing.
Aliquot store at -20°C or -80°C. Avoid repeated freeze /
Purity: Immunogen affinity purified
Fig: Western blot analysis on D3 cell lysates
using anti-Oct 4 monoclonal antibody.
1."Oct4 pseudogenes are transcribed in cancers."Suo
G., Han J., Wang X., Zhang J., Zhao Y., Zhao Y., Dai J.Biochem.
Biophys. Res. Commun. 337:1047-1051(2005)
2."Sumoylation of Oct4 enhances its stability, DNA binding,
and transactivation."Wei F., Schoeler H.R., Atchison M.L.J.
Biol. Chem. 282:21551-21560(2007)
3."Post-translational modification of POU domain
transcription factor Oct-4 by SUMO-1."Zhang Z., Liao B., Xu
M., Jin Y.FASEB J. 21:3042-3051(2007)